show Abstracthide AbstractVicia villosa is an incompletely domesticated annual legume of the Fabaceae family native to Europe and Western Asia. V. villosa is widely used as a cover crop in the United States Midwest due to an ability to withstand harsh winters. The highly heterozygous nature of the V. villosa assembly precluded prior genome assembly using short-reads, leaving this species without suitable genomic resources for genetic selection. A reference-quality genome assembly was prepared from low error rate long sequence reads to improve genetic-based trait selection of this species. This reference genome assembly was further filtered for duplicate sequences and was scaffolded using Hi-C reads to provide the first contiguous assembly for the species.